EWS::FLI1 and HOXD13 Control Tumor Cell Plasticity in Ewing Sarcoma.
Clin Cancer Res
; 28(20): 4466-4478, 2022 10 14.
Article
em En
| MEDLINE
| ID: mdl-35653119
ABSTRACT
PURPOSE:
Propagation of Ewing sarcoma requires precise regulation of EWSFLI1 transcriptional activity. Determining the mechanisms of fusion regulation will advance our understanding of tumor progression. Here we investigated whether HOXD13, a developmental transcription factor that promotes Ewing sarcoma metastatic phenotypes, influences EWSFLI1 transcriptional activity. EXPERIMENTALDESIGN:
Existing tumor and cell line datasets were used to define EWSFLI1 binding sites and transcriptional targets. Chromatin immunoprecipitation and CRISPR interference were employed to identify enhancers. CUT&RUN and RNA sequencing defined binding sites and transcriptional targets of HOXD13. Transcriptional states were investigated using bulk and single-cell transcriptomic data from cell lines, patient-derived xenografts, and patient tumors. Mesenchymal phenotypes were assessed by gene set enrichment, flow cytometry, and migration assays.RESULTS:
We found that EWSFLI1 creates a de novo GGAA microsatellite enhancer in a developmentally conserved regulatory region of the HOXD locus. Knockdown of HOXD13 led to widespread changes in expression of developmental gene programs and EWSFLI1 targets. HOXD13 binding was enriched at established EWSFLI1 binding sites where it influenced expression of EWSFLI1-activated genes. More strikingly, HOXD13 bound and activated EWSFLI1-repressed genes, leading to adoption of mesenchymal and migratory cell states that are normally suppressed by the fusion. Single-cell analysis confirmed that direct transcriptional antagonism between HOXD13-mediated gene activation and EWSFLI1-dependent gene repression defines the state of Ewing sarcoma cells along a mesenchymal axis.CONCLUSIONS:
Ewing sarcoma tumors are comprised of tumor cells that exist along a mesenchymal transcriptional continuum. The identity of cells along this continuum is, in large part, determined by the competing activities of EWSFLI1 and HOXD13. See related commentary by Weiss and Bailey, p. 4360.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sarcoma de Ewing
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article