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Chromatin behavior in living cells: Lessons from single-nucleosome imaging and tracking.
Ide, Satoru; Tamura, Sachiko; Maeshima, Kazuhiro.
Afiliação
  • Ide S; Genome Dynamics Laboratory, National Institute of Genetics, ROIS, Mishima, Shizuoka, Japan.
  • Tamura S; Department of Genetics, School of Life Science, SOKENDAI, Mishima, Shizuoka, Japan.
  • Maeshima K; Genome Dynamics Laboratory, National Institute of Genetics, ROIS, Mishima, Shizuoka, Japan.
Bioessays ; 44(7): e2200043, 2022 07.
Article em En | MEDLINE | ID: mdl-35661389
Eukaryotic genome DNA is wrapped around core histones and forms a nucleosome structure. Together with associated proteins and RNAs, these nucleosomes are organized three-dimensionally in the cell as chromatin. Emerging evidence demonstrates that chromatin consists of rather irregular and variable nucleosome arrangements without the regular fiber structure and that its dynamic behavior plays a critical role in regulating various genome functions. Single-nucleosome imaging is a promising method to investigate chromatin behavior in living cells. It reveals local chromatin motion, which reflects chromatin organization not observed in chemically fixed cells. The motion data is like a gold mine. Data analyses from many aspects bring us more and more information that contributes to better understanding of genome functions. In this review article, we describe imaging of single-nucleosomes and their tracked behavior through oblique illumination microscopy. We also discuss applications of this technique, especially in elucidating nucleolar organization in living cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatina / Nucleossomos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatina / Nucleossomos Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article