Role of PGC-1α in fiber type conversion in the palatopharyngeus muscle of OSA patients.
J Clin Lab Anal
; 36(7): e24551, 2022 Jul.
Article
em En
| MEDLINE
| ID: mdl-35692078
ABSTRACT
BACKGROUND:
Obstructive sleep apnea (OSA) has a high incidence and is harmful to health. It is characterized by repeated collapse of the upper airway. However, the mechanism underlying upper airway collapse is unclear.METHODS:
Patients with OSA and chronic tonsillitis were studied. Pathological changes in palatopharyngeus muscle were detected. The expression of peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) and nuclear respiratory factor-1 (NRF-1) in muscles was detected by PCR and Western blotting. Immunofluorescence staining was used to detect the expression of type I and type II myofibril.RESULTS:
The structure of the palatopharyngeus muscle was changed, and the expression of PGC-1α and NRF-1 was decreased in the OSA group compared with that in the control group. The expression of PGC-1α, NRF-1, and type I myofibril in C2C12 myoblasts was decreased by intermittent hypoxia exposure. The expression of type I myofibril was decreased when knocking down PGC-1α.CONCLUSION:
OSA patients exhibited pathological damage in palatopharyngeus muscle. PGC-1α was involved in the fiber type conversion in palatopharyngeus muscle caused by intermittent hypoxia.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Músculos Faríngeos
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Apneia Obstrutiva do Sono
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Fator 1 Nuclear Respiratório
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Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article