A single allele of the hsa-miR-302/367 cluster maintains human pluripotent stem cells.

Sugawara, Tohru; Kawamoto, Yuki; Kawasaki, Tomoyuki; Umezawa, Akihiro; Akutsu, Hidenori

Sugawara, Tohru; Kawamoto, Yuki; Kawasaki, Tomoyuki; Umezawa, Akihiro; Akutsu, Hidenori.

Afiliação

Sugawara T; Center for Regenerative Medicine, National Center for Child Health and Development (NCCHD), Tokyo, 157-8535, Japan.
Kawamoto Y; Center for Regenerative Medicine, National Center for Child Health and Development (NCCHD), Tokyo, 157-8535, Japan.
Kawasaki T; Center for Regenerative Medicine, National Center for Child Health and Development (NCCHD), Tokyo, 157-8535, Japan.
Umezawa A; Center for Regenerative Medicine, National Center for Child Health and Development (NCCHD), Tokyo, 157-8535, Japan.
Akutsu H; Center for Regenerative Medicine, National Center for Child Health and Development (NCCHD), Tokyo, 157-8535, Japan.

Regen Ther ; 21: 37-45, 2022 Dec.

Article em En | MEDLINE | ID: mdl-35702483

Palavras-chave

CRISPR, clustered regularly interspaced short palindromic repeats; CRISPR/Cas9; EB, embryoid body; EGFP, enhanced green fluorescent protein; LHA, left homologous arm; NSC, neural stem cell; PSC, pluripotent stem cell; Poly(A) signal, polyadenylation signal; RFP, red fluorescent protein; RHA, right homologous arm; TALE, transcription activator-like effector; hetKO, hsa-miR-302/367 cluster heterozygous knockout; iPS cells; miR-302; miRs, microRNAs; microRNAs; tRFP, TurboRFP

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article