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Therapeutic Benefits of Pomegranate Flower Extract: A Novel Effect That Reduces Oxidative Stress and Significantly Improves Diastolic Relaxation in Hyperglycemic In Vitro in Rats.
Wei, Yuanyuan; Khalaf, Ahmad Taha; Ye, Peng; Fan, Wei; Su, Junyi; Chen, Wanlu; Hu, Hao; Menhas, Rashid; Wang, Lifeng; Oglah, Zahraa.
Afiliação
  • Wei Y; College of Medicine, Chengdu University, Chengdu 610106, Sichuan, China.
  • Khalaf AT; College of Medicine, Chengdu University, Chengdu 610106, Sichuan, China.
  • Ye P; Department of Anatomy and Histology, College of Medicine, Chengdu University, Chengdu 610106, Sichuan, China.
  • Fan W; School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610106, Sichuan, China.
  • Su J; College of Medical, Fujian Medical University, Fuzhou 350108, Fujian, China.
  • Chen W; Department of Anatomy and Histology, College of Medicine, Chengdu University, Chengdu 610106, Sichuan, China.
  • Hu H; Department of Anatomy and Histology, College of Medicine, Chengdu University, Chengdu 610106, Sichuan, China.
  • Menhas R; Research Center of Sport Social Sciences, School of Physical Education and Sports, Soochow University, Suzhou, Jiangsu, China.
  • Wang L; Department of Physiology, Xinjiang Medical University, Urumqi 830011, Xinjiang, China.
  • Oglah Z; School of Science, Auckland University of Technology (AUT), 55 Wellesley Street East, Auckland, New Zealand.
Article em En | MEDLINE | ID: mdl-35722136
The pomegranate flower is an ancient herb in traditional Chinese medicine with multiple properties. Recent studies have shown that pomegranate flower extract is beneficial, especially for hyperglycemia. In this experiment, we investigated the diastolic effect of pomegranate flower polyphenol (PFP) extract on the isolated thoracic aorta of rats in both the absence and presence of high glucose levels. Isotonic contractile forces were recorded from aortic rings (about 3 mm in length) from rats using the BL-420F Biological Function Test System. Tissues were precontracted with 60 mM KCl to obtain maximum tension under 1.0 g load for 1 hour before the balance was achieved, and the fluid was changed every 15 minutes. PFP (700 mg/L-900 mg/L) showed a concentration-dependent relaxant effect on the aortic rings; vasodilation in the endothelium-intact was significantly higher than that in the de-endothelialized segments (P < 0.01). The endothelium-dependent vasorelaxant effect of PFP was partially attenuated by K+ channel blockers, tetraethylammonium (TEA), glibenclamide (Glib), and BaCl2, as well as L-NAME (eNOS inhibitor) on the denuded endothelium artery ring. Concentration-dependent inhibition of PFP on releasing intracellular Ca2+ in the Ca2+-free solution and vasoconstriction of CaCl2 in Ca2+-free buffer plus K+ (60 mM) was observed. In addition, PFP (0.1-10 mg/L) showed significant inhibition of acetylcholine-induced endothelial-dependent relaxation in the aorta of rats in the presence of high glucose (44 mmol/L). Nevertheless, the vasodilating effect of PFP was inhibited by atropine and L-NAME. The results indicated that PFP-induced vasodilation was most likely related to the antioxidant effects through enhanced NO synthesis, as well as the blocking of K+ channels and inhibition of extracellular Ca2+ entry. In conclusion, these observations showed that PFP ameliorates vasodilation in hyperglycemic rats. Hence, our results suggest that PFP supplementation may be beneficial for hypertensive patients with diabetes.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article