Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4.
Appl Microbiol Biotechnol
; 106(12): 4617-4626, 2022 Jun.
Article
em En
| MEDLINE
| ID: mdl-35739346
ABSTRACT
Zymomonas mobilis ZM4 is an attractive host for the development of microbial cell factories to synthesize high-value compounds, including prebiotics. In this study, a straightforward process to produce fructooligosaccharides (FOS) from sucrose was established. To control the relative FOS composition, recombinant Z. mobilis strains secreting a native levansucrase (encoded by sacB) or a mutated ß-fructofuranosidase (Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both strains were able to produce a FOS mixture with high concentration of 6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB that was able to produce 73.4 ± 1.6 g L-1 of FOS, with a productivity of 1.53 ± 0.03 g L-1 h-1 and a yield of 0.31 ± 0.03 gFOS gsucrose-1. This is the first report on the FOS production using a mutant Z. mobilis ZM4 strain in a one-step process. KEY POINTS ⢠Zymomonas mobilis was engineered to produce FOS in a one-step fermentation process. ⢠Mutant strains produced FOS mixtures with high concentration of 6-kestose. ⢠A new route to produce tailor-made FOS mixtures was presented.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zymomonas
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article