Your browser doesn't support javascript.
loading
Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions.
Simonová, Anna; Romanská, Veronika; Benoni, Barbora; Skubník, Karel; Smerdová, Lenka; Procházková, Michaela; Spustová, Kristina; Moravcík, Ondrej; Gahurova, Lenka; Paces, Jan; Plevka, Pavel; Cahová, Hana.
Afiliação
  • Simonová A; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic.
  • Romanská V; First Faculty of Medicine Charles University, Katerinská 32, 12108, Prague 2, Czech Republic.
  • Benoni B; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic.
  • Skubník K; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic.
  • Smerdová L; First Faculty of Medicine Charles University, Katerinská 32, 12108, Prague 2, Czech Republic.
  • Procházková M; Structural Virology, Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
  • Spustová K; Structural Virology, Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
  • Moravcík O; Structural Virology, Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
  • Gahurova L; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic.
  • Paces J; Institute of Molecular Genetics of the Czech Academy of Sciences, 14220, Prague, Czech Republic.
  • Plevka P; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague 6, Czech Republic.
  • Cahová H; Department of Molecular Biology and Genetics, Faculty of Science, University of South Bohemia, Branisovska 1760, 37005, Ceské Budejovice, Czech Republic.
Chembiochem ; 23(17): e202200281, 2022 09 05.
Article em En | MEDLINE | ID: mdl-35771148
The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles; thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC-MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N6 -methyladenosine and 2'-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m1 A) and 5-methylcytidine (m5 C). Mapping of m1 A and m5 C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3' and 5' fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA de Transferência / Nucleosídeos Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA de Transferência / Nucleosídeos Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article