Re-engineering of CUP1 promoter and Cup2/Ace1 transactivator to convert Saccharomyces cerevisiae into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copper.

Zunar, Bojan; Mosrin, Christine; Bénédetti, Héléne; Vallée, Béatrice

Re-engineering of CUP1 promoter and Cup2/Ace1 transactivator to convert Saccharomyces cerevisiae into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copper.

Zunar, Bojan; Mosrin, Christine; Bénédetti, Héléne; Vallée, Béatrice.

Afiliação

Zunar B; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071, Orléans Cedex 2, France; Laboratory for Biochemistry, Department of Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia.
Mosrin C; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071, Orléans Cedex 2, France.
Bénédetti H; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071, Orléans Cedex 2, France.
Vallée B; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071, Orléans Cedex 2, France. Electronic address: beatrice.vallee@cnrs-orleans.fr.

Biosens Bioelectron ; 214: 114502, 2022 Oct 15.

Article em En | MEDLINE | ID: mdl-35785751

Assuntos

Técnicas Biossensoriais; Metais Pesados; Técnicas Biossensoriais/métodos; Cobre; Humanos; Metalotioneína; Saccharomyces cerevisiae/genética; Transativadores

Palavras-chave

Bioavailability; Copper biosensor; Saccharomyces cerevisiae; Transactivator engineering; Whole-cell biosensor

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Metais Pesados Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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