Your browser doesn't support javascript.
loading
Chlorogenic acid suppresses miR-460a in the regulation of Bcl-2, causing interleukin-1ß reduction in thiram exposed chondrocytes via caspase-3/caspase-7 pathway.
Kulyar, Muhammad Fakhar-E-Alam; Mo, Quan; Yao, Wangyuan; Ding, Yanmei; Yan, Zhang; Du, Haitao; Pan, Huachun; Li, Kewei; Gao, Jindong; Shahzad, Muhammad; Mansoor, Muhammad Khalid; Iqbal, Mudassar; Waqas, Muhammad; Akhtar, Muhammad; Bhutta, Zeeshan Ahmad; Li, Jiakui.
Afiliação
  • Kulyar MF; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Mo Q; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Yao W; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Microbiology and Plant Pathology, University of California-Riverside, Riverside, CA 92521, USA.
  • Ding Y; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Yan Z; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Du H; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Pan H; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Li K; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Gao J; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Shahzad M; Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan.
  • Mansoor MK; Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan.
  • Iqbal M; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China; Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, 63100, Pakistan.
  • Waqas M; Faculty of Veterinary & Animal Sciences, University of Poonch Rawalakot, Azad Jammu & Kashmir, 12350, Pakistan.
  • Akhtar M; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
  • Bhutta ZA; College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
  • Li J; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China; Department of Microbiology and Plant Pathology, University of California-Riverside, Riverside, CA 92521, USA. Electronic address: lijk210@sina.com.
Phytomedicine ; 104: 154296, 2022 Sep.
Article em En | MEDLINE | ID: mdl-35809377
ABSTRACT

BACKGROUND:

Apoptosis is thought to be involved in all processes, including normal cell cycle, immune system, atrophy, embryonic development, and chemical-induced cellular damage. However, if the normal apoptotic process fails, the results might be disastrous, e.g., chondrocytes damage in tibial dyschondroplasia (TD). TD is a worldwide issue in the poultry sector due to thiram toxicity. Thiram (Tetramethyl thiuram disulfide) is a dithiocarbamate pesticide and fungicide commonly used in horticulture to treat grains meant for seed protection and preservation.

PURPOSE:

According to prior studies, chlorogenic acid (CGA) is becoming essential for regulating apoptosis. But still, the specific role of CGA in chondrocyte cells remains unclear. The present study explored the molecular mechanism of CGA on chondrocytes' apoptosis with B-cell lymphoma 2 signaling under the effect of miR-460a.

METHODS:

An in vivo and in vitro study was performed according to our previously developed methodology. Flow cytometry, western blotting, reverse transcription-quantitative polymerase chain reaction, and immunofluorescence assay were used to investigate the involvement of apoptosis and inflammasome related pathways.

RESULTS:

The CGA decreased the apoptosis rate with the deactivation of miR-460a, accompanied by the activation of Bcl-2. The high expression of miR-460a reduced the cell viability of chondrocytes in vitro and in vivo, that led to the interleukin-1ß production. While the apoptotic executioners (caspase-3 and caspase-7) acted upstream in miR-460a overexpressing cells, and its depletion downgraded these executioners. The CGA administrated cells negatively regulated miR-460a expression and thus indicating the deactivation of the apoptotic and inflammasome related pathways.

CONCLUSION:

Chlorogenic acid had a negative effect on miR-460a, setting off specific feedback to regulate apoptotic and inflammasome pathways, which might be a key feature for chondrocytes' survival.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteocondrodisplasias / MicroRNAs Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteocondrodisplasias / MicroRNAs Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article