Magnetic covalent-organic frameworks-based extraction followed by UHPLC-MS/MS for determination and pharmacokinetic study of trace angoroside C in rat plasma after oral administration of Xuanbo Shuangsheng Granule.

The composition of the traditional Chinese medicine compound preparation is complex, while the content of each active ingredient is extremely low, which brings difficulties to the plasma concentration detection. In this study, the magnetic covalent-organic frameworks were synthesized by a simple one-step Schiff base reaction and applied for the specific extraction of trace angoroside C in rat plasma prior to ultra-high-performance liquid chromatography-tandem mass spectrometry detection. The synthesized magnetic covalent-organic frameworks have high magnetic responsiveness (35.67 emu·g-1 ), large surface area (110.9 m2 ·g-1 ), and strong stability. The as-prepared material can quickly extract angoroside C from plasma with high extraction efficiency, be easily separated with a magnet afterward, and can be reused for at least five times. The established method was systematically validated showing good linearity (0.1-5 ng·ml-1 ), low limit of quantification (0.1 ng·ml-1 ), good accuracy (93.18-105.36%), and good precision (percentage relative standard deviation 3.60-10.90%). Finally, the method was used to the pharmacokinetic study of trace angoroside C in rats after oral administration of Xuanbo Shuangsheng Granule.