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Green tea epigallocatechin gallate suppresses 3T3-L1 cell growth via microRNA-143/MAPK7 pathways.
Chen, Chia-Pei; Su, Tsung-Chen; Yang, Meei-Ju; Chen, Wen-Ting; Siao, An-Ci; Huang, Ling-Ru; Lin, Yen-Yue; Kuo, Yow-Chii; Chung, Jia-Fang; Cheng, Ching-Feng; Ku, Hui-Chen; Kao, Yung-Hsi.
Afiliação
  • Chen CP; Department of Life Sciences, National Central University, Taoyuan 320.
  • Su TC; Tea Research and Extension Station, Council of Agriculture, Taoyuan 326.
  • Yang MJ; Tea Research and Extension Station, Council of Agriculture, Taoyuan 326.
  • Chen WT; Department of Life Sciences, National Central University, Taoyuan 320.
  • Siao AC; Department of Life Sciences, National Central University, Taoyuan 320.
  • Huang LR; Department of Life Sciences, National Central University, Taoyuan 320.
  • Lin YY; Department of Life Sciences, National Central University, Taoyuan 320.
  • Kuo YC; Department of Emergency Medicine, Taoyuan Armed Forces General Hospital, Taoyuan 325.
  • Chung JF; Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114.
  • Cheng CF; Department of Gastroenterology, Landseed Hospital, Taoyuan 324.
  • Ku HC; Department of Pediatrics, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City 23142.
  • Kao YH; Department of Pediatrics, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City 23142.
Exp Biol Med (Maywood) ; 247(18): 1670-1679, 2022 09.
Article em En | MEDLINE | ID: mdl-35894140
Green tea epigallocatechin gallate (EGCG) and microRNA (miRNA) molecules modulate obesity. Nevertheless, it is still unknown whether EGCG modulates fat cell growth via miRNA-related signaling. In this study, white preadipocytes were used to examine whether the antimitogenic effect of EGCG on fat cells is regulated by the miR-143/MAPK7 pathway. We showed that EGCG upregulated the levels of miR-143, but not miR-155, in 3T3-L1 preadipocytes. Moreover, EGCG downregulated MAPK7 mRNA and protein levels time- and dose-dependently. MAPK7 expression increased during 3T3-L1 cell proliferation. miR-143 overexpression in the absence of EGCG mimicked the effects of EGCG to suppress preadipocyte growth and MAPK7 expression, whereas knockdown of miR-143 antagonized the EGCG-altered levels of miR-143, MAPK7, and pERK1/2 and reversed the EGCG-inhibited cell growth. These findings suggest that EGCG inhibits 3T3-L1 cell growth via miR-143/MAPK7 pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Catequina / MicroRNAs Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Catequina / MicroRNAs Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article