Your browser doesn't support javascript.
loading
A novel identified circular RNA, circSnap47, promotes heart failure progression via regulation of miR-223-3p/MAPK axis.
Wang, Yunkai; Wang, Hongqiang; Zhang, Luping; Zhang, Jinhua; Liu, Ning; Zhao, Peng.
Afiliação
  • Wang Y; Department of Cardiac Surgery ICU, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, People's Republic of China.
  • Wang H; Department of Cardiac Surgery II, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, People's Republic of China.
  • Zhang L; Department of Reproductive Medicine, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, People's Republic of China.
  • Zhang J; Department of Physical Examination, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, People's Republic of China.
  • Liu N; Department I of Obstetrics, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, People's Republic of China.
  • Zhao P; Department of Cardiac Surgery I, Yantai Yuhuangding Hospital, No.20 Yuhuangding East Road, Zhifu District, Yantai, 264000, Shandong, People's Republic of China. pengzhao198611@163.com.
Mol Cell Biochem ; 478(3): 459-469, 2023 Mar.
Article em En | MEDLINE | ID: mdl-35900666
The aim of this study was to investigate the effect of circSnap47 on heart failure (HF) and its potential mechanisms. Quantitative real-time PCR (qRT-PCR) was performed to detect the mRNA expression levels of circSnap47 and miR-233-3p. The viability and apoptosis of H9C2 cells were assessed using CCK-8 and TUNEL assays. The expressions of interleukin (IL)-6, IL-1ß, IL-18, and tumor necrosis factor-alpha were determined using ELISA and qRT-PCR. In addition, the expression of apoptosis-related proteins and mitogen-activated protein kinase (MAPK) signaling pathway-related proteins was analyzed using western blot. Moreover, HF-related circRNAs and miRNAs were predicted via bioinformatics analysis. The relationship between circSnap47 and miR-233-3p was further confirmed using a dual-luciferase reporter gene assay. In HF tissues and H9C2 cells treated with oxygen-glucose deprivation (OGD), circSnap47 was upregulated. Silencing circSnap47 increased cell viability and inhibited apoptosis. Besides, silencing circSnap47 alleviated OGD-induced inflammation in H9C2 cells. Moreover, we found that miR-233-3p was the downstream target gene of circSnap47. Our results also revealed that silencing circSnap47 relieved OGD-induced H9C2 cell damage by inactivating the miR-223-3p/MAPK axis. We confirmed that circSnap47 silencing inhibited HF progression via regulation of miR-223/MAPK axis, which will provide for a new therapeutic direction for the treatment of HF.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs / RNA Circular / Insuficiência Cardíaca Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: MicroRNAs / RNA Circular / Insuficiência Cardíaca Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article