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Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography.
Zhang, Meng; Díaz-Celis, César; Onoa, Bibiana; Cañari-Chumpitaz, Cristhian; Requejo, Katherinne I; Liu, Jianfang; Vien, Michael; Nogales, Eva; Ren, Gang; Bustamante, Carlos.
Afiliação
  • Zhang M; Applied Science and Technology Graduate Group, University of California, Berkeley, CA, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, USA; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Díaz-Celis C; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.
  • Onoa B; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.
  • Cañari-Chumpitaz C; Department of Chemistry, University of California, Berkeley, CA, USA.
  • Requejo KI; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.
  • Liu J; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Vien M; Department of Physics, University of California, Berkeley, CA, USA.
  • Nogales E; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA; Molecular Biophysics and Integrative Bi
  • Ren G; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Electronic address: gren@lbl.gov.
  • Bustamante C; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA; Department of Chemistry, University of California, Berkeley, CA, USA; Department of Physics, University of California, Berkele
Mol Cell ; 82(16): 3000-3014.e9, 2022 08 18.
Article em En | MEDLINE | ID: mdl-35907400
It has been proposed that the intrinsic property of nucleosome arrays to undergo liquid-liquid phase separation (LLPS) in vitro is responsible for chromatin domain organization in vivo. However, understanding nucleosomal LLPS has been hindered by the challenge to characterize the structure of the resulting heterogeneous condensates. We used cryo-electron tomography and deep-learning-based 3D reconstruction/segmentation to determine the molecular organization of condensates at various stages of LLPS. We show that nucleosomal LLPS involves a two-step process: a spinodal decomposition process yielding irregular condensates, followed by their unfavorable conversion into more compact, spherical nuclei that grow into larger spherical aggregates through accretion of spinodal materials or by fusion with other spherical condensates. Histone H1 catalyzes more than 10-fold the spinodal-to-spherical conversion. We propose that this transition involves exposure of nucleosome hydrophobic surfaces causing modified inter-nucleosome interactions. These results suggest a physical mechanism by which chromatin may transition from interphase to metaphase structures.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nucleossomos / Tomografia com Microscopia Eletrônica Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nucleossomos / Tomografia com Microscopia Eletrônica Idioma: En Ano de publicação: 2022 Tipo de documento: Article