Nanostructured Cyclodextrin-Mediated Surface for Capacitive Determination of Cortisol in Multiple Biofluids.
ACS Appl Mater Interfaces
; 14(37): 42374-42387, 2022 Sep 21.
Article
em En
| MEDLINE
| ID: mdl-35918826
ABSTRACT
The aim of this work is to develop a reusable polypropylene glycol (PPG)ß-cyclodextrin (ßCD) biosensor for cortisol detection. To achieve the most stable support for ßCD, we developed two PPG surfaces. The first surface is based on a gold surface modified with SAM of 3-mercaptopropionic acid (3MPA), and the second surface is based on a glassy carbon surface grafted with 4-carboxyphenyl diazonium salt. We characterized both surfaces by EIS, XPS, and ATR-FTIR and evaluated the stability and reusability of each surface. We found the GC-carboxyphenyl-PPGßCD is stable for at least 1 month. We have also demonstrated the reusability of the surface up to 10 times. In detecting cortisol, we used a nonfaradaic electrochemical impedance capacitive model to interpret the surface confirmation changes. We achieved sensitive detection of cortisol in PBS buffer, urine, and saliva with limit of detection of 2.13, 1.29, and 1.33 nM, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Ciclodextrinas
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Beta-Ciclodextrinas
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article