Fluorescence ratiometric assay for discriminating GSH and Cys based on the composites of UiO-66-NH2 and Cu nanoclusters.
Biosens Bioelectron
; 215: 114582, 2022 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-35933738
ABSTRACT
The discriminative detection of glutathione (GSH) from cysteine (Cys) remains a challenge because of their similarity in structure and chemical properties. This study reported a strategy for selective and sensitive detection of GSH based on the GSH-promoted blue fluorescence of UiO-66-NH2 and aggregation-enhanced emission (AEE) feature of orange emissive Cu nanoclusters (NCs). A relatively weak blue fluorescence of UiO-66-NH2 was converted to strong after reacting with GSH due to the rotation-restricted emission enhancement mechanism. In addition, the GSH-activated UiO-66-NH2 was further used as a template and reducing reagent for synthesizing orange-red AEE active Cu NCs composites (UiO-66-NH2@Cu NCs). A ratiometric fluorescence response was observed after forming UiO-66-NH2@Cu NCs, helping discriminate GSH over Cys. In addition, UiO-66-NH2@Cu NCs were further utilized for the detection of GSH in clinical samples. The present findings provide an efficient strategy to discriminate GSH over Cys and open a new door for utilizing and functionalizing metal-organic frameworks (MOFs) for various applications.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Nanopartículas Metálicas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article