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Mammaglobin 1 mediates progression of trastuzumab-resistant breast cancer cells through regulation of cyclins and NF-κB.
Kusumastuti, Ratih; Kumagai, Yuji; Ishihara, Seiichiro; Enomoto, Atsushi; Murakami, Takashi; Yasuda, Motoaki; Haga, Hisashi.
Afiliação
  • Kusumastuti R; Division of Life Science, Graduate School of Life Science, Hokkaido University, Sapporo, Japan.
  • Kumagai Y; Division of Life Science, Graduate School of Life Science, Hokkaido University, Sapporo, Japan.
  • Ishihara S; Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
  • Enomoto A; Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
  • Murakami T; Faculty of Medicine, Saitama Medical University, Moroyama, Japan.
  • Yasuda M; Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
  • Haga H; Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
FEBS Open Bio ; 12(10): 1797-1813, 2022 10.
Article em En | MEDLINE | ID: mdl-35945910
Overexpression of human epidermal growth factor receptor 2 (HER2) in various cancers is correlated with poor patient survival. Trastuzumab, a recombinant humanized monoclonal antibody against HER2, has been considered to be a first-line therapy for HER2-positive breast cancer patients, but its usefulness is limited by the development of resistance. In this study, we established resistant cells by long-term treatment with trastuzumab. These cells showed higher proliferation, invasion, and migration abilities than the wild-type cells. Mammaglobin 1 (MGB1), cyclin D1, E1, A2, and phosphorylated NF-κB (p-p65) were upregulated in resistant cells. These proteins regulate cell proliferation, migration, and invasion of resistant cells. Depletion of MGB1 decreased cyclin and p-p65 expression. Cyclin D1 and A2, but not E1 expression, were affected by p-p65 downregulation. In summary, our results indicate that MGB1 expression is increased in breast cancer cells that have gained resistance to trastuzumab, and suggest that MGB1 promotes aggressiveness through cyclin and NF-κB regulation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / NF-kappa B Limite: Female / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / NF-kappa B Limite: Female / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article