Mixotrophic Cultivation Optimization of Microalga Euglena pisciformis AEW501 for Paramylon Production.
Mar Drugs
; 20(8)2022 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-36005522
ABSTRACT
Euglena, a flagellated unicellular protist, has recently received widespread attention for various high-value metabolites, especially paramylon, which was only found in Euglenophyta. The limited species and low biomass of Euglena has impeded paramylon exploitation and utilization. This study established an optimal cultivation method of Euglena pisciformis AEW501 for paramylon production under mixotrophic cultivation. The results showed that the optimum mixotrophic conditions were 20 °C, pH 7.0, and 63 µmol photons m-2âs-1, and the concentrations of sodium acetate and diammonium hydrogen phosphate were 0.98 g L-1 and 0.79 g L-1, respectively. The maximal biomass and paramylon content were 0.72 g L-1 and 71.39% of dry weight. The algal powder contained more than 16 amino acids, 6 vitamins, and 10 unsaturated fatty acids under the optimal cultivation. E. pisciformis paramylon was pure ß-1,3-glucan-type polysaccharide (the purity was up to 99.13 ± 0.61%) composed of linear glucose chains linked together by ß-1,3-glycosidic bonds. These findings present a valuable basis for the industrial exploitation of paramylon with E. pisciformis AEW501.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Euglena
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Euglena gracilis
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Microalgas
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article