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Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells.
Wei, Yanxing; Xiao, Lu; Ma, Lishi; Wang, Zhijian; Huang, Liping; Li, Huiying; Pan, Guangjin; Lye, Stephen J; Shan, Yongli.
Afiliação
  • Wei Y; Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5T3H7, Canada; Key Laboratory of Functional Proteomics of Guangdong Province, School of
  • Xiao L; Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Ma L; Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Wang Z; Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Huang L; Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Li H; The First Clinical Medical School, Southern Medical University, Guangzhou 510515, China.
  • Pan G; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
  • Lye SJ; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5T3H7, Canada; Department of Obstetrics & Gynecology, University of Toronto, Toronto, ON M5G1L4, Canada; Department of Physiology, University of Toronto, Toronto, ON M5G1L4, Canada.
  • Shan Y; Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health,
STAR Protoc ; 3(3): 101638, 2022 09 16.
Article em En | MEDLINE | ID: mdl-36042882
ABSTRACT
Human trophoblast stem cells (hTSCs) are useful for studying human placenta development and diseases, but primed human pluripotent stem cells (hPSCs) routinely cultured in most laboratories do not support hTSC derivation. Here, we present a protocol to derive hTSCs directly from primed hPSCs. This approach, containing two strategies either with or without bone morphogenetic protein 4 (BMP4), provides a simple and accessible tool for deriving hTSCs to study placenta development and disease modeling without ethical limitations or reprogramming process. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trofoblastos / Células-Tronco Pluripotentes Limite: Female / Humans / Pregnancy Idioma: En Ano de publicação: 2022 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trofoblastos / Células-Tronco Pluripotentes Limite: Female / Humans / Pregnancy Idioma: En Ano de publicação: 2022 Tipo de documento: Article