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Glycogen Synthase Kinase-3 Isoform Variants and Their Inhibitory Phosphorylation in Human Testes and Spermatozoa.
Park, Seung Hyun; Xu, Yang; Park, Yong-Seog; Seo, Ju Tae; Gye, Myung Chan.
Afiliação
  • Park SH; Department of Life Science and Institute for Natural Sciences, Hanyang University, Seoul, Korea.
  • Xu Y; Department of Life Science and Institute for Natural Sciences, Hanyang University, Seoul, Korea.
  • Park YS; Laboratory of Reproductive Medicine, Cheil General Hospital & Women's Healthcare Center, Dankook University College of Medicine, Seoul, Korea.
  • Seo JT; Department of Urology, Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine, Seoul, Korea. jtandro@naver.com.
  • Gye MC; Department of Life Science and Institute for Natural Sciences, Hanyang University, Seoul, Korea. mcgye@hanyang.ac.kr.
World J Mens Health ; 41(1): 215-226, 2023 Jan.
Article em En | MEDLINE | ID: mdl-36047078
PURPOSE: To clarify (phospho-) glycogen synthase kinase-3 (GSK3) isoform variants in the germline and soma of human testes and spermatozoa. MATERIALS AND METHODS: GSK3 isoform variants in normospermatogenic and Sertoli cell-only (SCO) testicular biopsies and spermatozoa were examined. RESULTS: In normospermatogenic testes, GSK3α and GSK3ß variants 1 and 2 different in low complexity region (LCR) were expressed and their levels were decreased in SCO testes. GSK3ß variant 3 was only expressed in SCO testes. GSK3ß as well as GSK3α, the dominant isoforms in testes were decreased in SCO testes. In normospermatogenic testes, GSK3ß were found in spermatogonia and markedly decreased in meiotic germ cells in which GSK3α was dominant. p-GSK3α/ß were marginal in spermatogonia and early spermatocytes. In SCO testes, GSK3α/ß immunoreactivity in seminiferous epithelia was weaker than those of normospermatogenic testes whereas p-GSK3α/ß(Ser) immunoreactivity was visibly increased in Sertoli cells. GSK3α was dominant in ejaculated spermatozoa in which GSK3α and p-GSK3α(Ser) were found in the head, midpiece, and tail. In acrosome-reacted spermatozoa, GSK3α was found in the equatorial region of head, midpiece, and tail, and p-GSK3α(Ser) was only found in midpiece. During sperm capacitation, p-GSK3α(Ser) was significantly increased together with phosphotyrosine proteins and motility. CONCLUSIONS: In human male germ cells, GSK3 isoforms different in LCRs switch from GSK3ß to GSK3α during meiotic entry, suggesting the isoform-specific roles of GSK3α and GSK3ß in meiosis and stemness or proliferation of spermatogonia, respectively. In dormant Sertoli cells of SCO testes kinase activity of GSK3 might be downregulated via inhibitory phosphorylation. In spermatozoa, inhibitory phosphorylation of GSK3α might be coupled with activation of motility during capacitation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article