Single-cell analyses of axolotl telencephalon organization, neurogenesis, and regeneration.
Science
; 377(6610): eabp9262, 2022 09 02.
Article
em En
| MEDLINE
| ID: mdl-36048956
ABSTRACT
Salamanders are tetrapod models to study brain organization and regeneration; however, the identity and evolutionary conservation of brain cell types are largely unknown. We delineated the cell populations in the axolotl telencephalon during homeostasis and regeneration using single-cell genomic profiling. We identified glutamatergic neurons with similarities to amniote neurons of hippocampus, dorsal and lateral cortex, and conserved γ-aminobutyric acid-releasing (GABAergic) neuron classes. We inferred transcriptional dynamics and gene regulatory relationships of postembryonic, region-specific neurogenesis and unraveled conserved differentiation signatures. After brain injury, ependymoglia activate an injury-specific state before reestablishing lost neuron populations and axonal connections. Together, our analyses yield insights into the organization, evolution, and regeneration of a tetrapod nervous system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Telencéfalo
/
Evolução Biológica
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Neurogênese
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Ambystoma mexicanum
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Regeneração do Cérebro
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article