Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice.
STAR Protoc
; 3(3): 101644, 2022 09 16.
Article
em En
| MEDLINE
| ID: mdl-36052343
ABSTRACT
Intratumor and gut mycobiome is linked to pancreatic ductal adenocarcinoma (PDAC) tumorigenesis; however, an optimal approach to culture and transplant fungus into mouse for in vivo studies is missing. This protocol describes culture steps of Alternaria alternata and Malassezia globosa and their subsequent transplantation into a PDAC mouse model via oral gavage. The utilization of the fungal culture method will allow for consistent growth and expansion of specific fungal species for downstream processing. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Carcinoma Ductal Pancreático
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article