Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection.
Vet Res
; 53(1): 67, 2022 Sep 02.
Article
em En
| MEDLINE
| ID: mdl-36056449
ABSTRACT
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Camelídeos Americanos
/
Infecções por Coronavirus
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Coronavírus da Síndrome Respiratória do Oriente Médio
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article