Localization of Multiple O-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
J Proteome Res
; 21(10): 2462-2471, 2022 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-36074808
ABSTRACT
We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms. We detected a wide range of abundances of various O-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry. To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows. In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer. Sixty-nine O-glycopeptides of the long O-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicopeptídeos
/
Espectrometria de Massas em Tandem
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article