Enriching and Characterizing T Cell Repertoires from 3' Barcoded Single-Cell Whole Transcriptome Amplification Products.

Jivanjee, Tasneem; Ibrahim, Samira; Nyquist, Sarah K; Gatter, G James; Bromley, Joshua D; Jaiswal, Swati; Berger, Bonnie; Behar, Samuel M; Love, J Christopher; Shalek, Alex K

Jivanjee, Tasneem; Ibrahim, Samira; Nyquist, Sarah K; Gatter, G James; Bromley, Joshua D; Jaiswal, Swati; Berger, Bonnie; Behar, Samuel M; Love, J Christopher; Shalek, Alex K.

Afiliação

Jivanjee T; Institute for Medical Engineering & Science and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
Ibrahim S; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nyquist SK; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Gatter GJ; Broad Institute of MIT and Harvard, Cambridge, MA, Massachusetts Institute of Technology, Cambridge, MA, USA.
Bromley JD; Institute for Medical Engineering & Science and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
Jaiswal S; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
Berger B; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA.
Behar SM; Broad Institute of MIT and Harvard, Cambridge, MA, Massachusetts Institute of Technology, Cambridge, MA, USA.
Love JC; Institute for Medical Engineering & Science and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
Shalek AK; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

Methods Mol Biol ; 2574: 159-182, 2022.

Article em En | MEDLINE | ID: mdl-36087201

Assuntos

Linfócitos T; Transcriptoma; Receptores de Antígenos de Linfócitos T/genética; Análise de Sequência de RNA/métodos; Análise de Célula Única/métodos

Palavras-chave

Gene expression; Seq-Well S3; Single-cell RNA-sequencing; Single-cell TCR sequencing; T cell receptor repertoire profiling; Targeted enrichment; scRNA-seq

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Transcriptoma Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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