Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins.
Methods Mol Biol
; 2564: 299-315, 2023.
Article
em En
| MEDLINE
| ID: mdl-36107350
ABSTRACT
Green fluorescent proteins (GFPs) have lightened up almost every aspect of biological research including protein sciences. In the field of membrane protein structural biology, GFPs have been used widely to monitor membrane protein localization, expression level, the purification process and yield, and the stability inside the cells and in the test tube. Of particular interest is the fluorescence-detector size-exclusion chromatography-based thermostability assay (FSEC-TS). By simple heating and FSEC, the generally applicable method allows rapid assessment of the thermostability of GFP-fused membrane proteins without purification. Here we describe the experimental details and some typical results for the FSEC-TS method.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article