A lateral flow immunoassay based on chemisorbed probes in virtue of hydrogen bond receptors on the Bi2S3 NPs.
Food Chem
; 401: 134133, 2023 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-36113217
ABSTRACT
Improving detection sensitivity is still a major research emphasis for lateral flow immunoassay (LFIA). Increasing the binding efficiency and stability of the probe is an achievable and effective solution. In this work, we developed a highly sensitive lateral flow immunoassay for clenbuterol detection by using bismuth sulfide nanoparticle (Bi2S3) nanoparticles (NPs) as a novel marker. Here, Bi2S3 NPs can link with the antibody by hydrogen bonding to improve the performance of the probe, e.g., stability and sensitivity. Benefiting from the direct hydrogen bonding between Bi2S3 NPs and the monoclonal antibody (mAb), high sensitivity is obtained by the proposed LFIA with a lower visible detection limit of 0.1 ng mL-1 and a cut-off value of 4 ng·mL-1 for CLE detection, which is 5-fold and 7.5-fold improved than the conventional Au NPs based LFIA. In addition, the encouraging practical application results in milk, pork, and beef show that the bismuth sulfide nanoparticle has a great popularizing potential in the performance promotion of LFIAs for food safety monitoring.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clembuterol
/
Nanopartículas
/
Nanopartículas Metálicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article