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Aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide for colorimetric identification of Salmonella typhimurium.
Wu, Pian; Huang, Ruixue; Chen, Cuimei; Yi, Jiecan; Kai, Tianhan; Zhan, Yujuan; Wei, Xiaoqian; Wang, Danqi; Zhang, Jingwen; Ding, Ping.
Afiliação
  • Wu P; Xiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China.
  • Huang R; Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, 410078, Hunan, China.
  • Chen C; Xiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China.
  • Yi J; Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, 410078, Hunan, China.
  • Kai T; School of Public Health, Xiang Nan University, Chenzhou, 423000, Hunan, China.
  • Zhan Y; School of Public Health, Changsha Medical University, Changsha, 410219, Hunan, China.
  • Wei X; Xiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China.
  • Wang D; Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, 410078, Hunan, China.
  • Zhang J; Xiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China.
  • Ding P; Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, 410078, Hunan, China.
Mikrochim Acta ; 189(11): 408, 2022 10 07.
Article em En | MEDLINE | ID: mdl-36205828
A novel aptamer-AuNP-conjugated carboxymethyl chitosan-functionalized graphene oxide (CMC/GO@Apt-Au NP) probe was for the first time developed for the determination of Salmonella typhimurium (S. typhimurium). Owing to the conformational change of the aptamers in the presence of S. typhimurium, the Au NPs, which were pre-adsorbed on the aptamers through van der Waals forces, were released into the solution phase and induced the color change of the solution. As a result, S. typhimurium ranging from 102 to 107 CFU/mL was successfully identified using the designed assay with a limit of detection (LOD) of 10 CFU/mL. This low detection level allowed the sensitive recognition of S. typhimurium in milk samples within 40 min without sample pretreatment, a conclusion that agreed well with the traditional plate counting method. The developed method not only provides a rapid way for the determination of S. typhimurium with simplicity and sensitivity but also shows potential universality in the quantification of other pathogenic microorganisms.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Aptâmeros de Nucleotídeos Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Aptâmeros de Nucleotídeos Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2022 Tipo de documento: Article