Label-Free Multimetric Measurement of Molecular Binding Kinetics by Electrical Modulation of a Flexible Nanobiolayer.
ACS Sens
; 7(11): 3461-3469, 2022 11 25.
Article
em En
| MEDLINE
| ID: mdl-36273329
Most label-free techniques rely on measuring refractive index or mass change on the sensor surface. Thus, it is challenging for them to measure small molecules or enzymatic processes that only induce a minor mass change on the analyte molecules. Here, we have developed a technique by combining Surface Plasmon Resonance sensing with an Oscillating Biomolecule Layer approach (SPR-OBL) to enhance the sensitivity of traditional SPR. In addition to the inherent mass sensitivity, SPR-OBL is also sensitive to the charge and conformational change of the analyte; hence it overcomes the mass limit and is able to detect small molecules. We show that the multimetric SPR-OBL measurement allows for sensing any changes regarding mass, charge, and conformation, which expands the detection capability of SPR.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Refratometria
/
Ressonância de Plasmônio de Superfície
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article