Cost-effective, high-yield production of <i>Pyrobaculum calidifontis</i> DNA polymerase for PCR application.

Maseh, Kashif; Ali, Syed Farhat; Ahmad, Shazeel; Rashid, Naeem

Cost-effective, high-yield production of Pyrobaculum calidifontis DNA polymerase for PCR application.

Maseh, Kashif; Ali, Syed Farhat; Ahmad, Shazeel; Rashid, Naeem.

Afiliação

Maseh K; KAM School of Life Sciences, Forman Christian College (A Chartered University), Lahore, Pakistan.
Ali SF; KAM School of Life Sciences, Forman Christian College (A Chartered University), Lahore, Pakistan.
Ahmad S; School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
Rashid N; School of Biological Sciences, University of the Punjab, Lahore, Pakistan.

Prep Biochem Biotechnol ; 53(6): 704-711, 2023.

Article em En | MEDLINE | ID: mdl-36306256

Assuntos

Pyrobaculum; Pyrobaculum/genética; Análise Custo-Benefício; Reação em Cadeia da Polimerase/métodos; DNA Polimerase Dirigida por DNA/química; DNA Polimerase Dirigida por DNA/metabolismo; Engenharia Genética; Escherichia coli/metabolismo

Palavras-chave

DNA polymerase; Pyrobaculum calidifontis; expression optimization; polymerase chain reaction (PCR); purification; stability

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pyrobaculum Tipo de estudo: Health_economic_evaluation Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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