Life History Metrics for Culex tarsalis (Diptera: Culicidae) and Culicoides sonorensis (Diptera: Ceratopogonidae) Are Not Impacted by Artificial Feeding on Defibrinated Versus EDTA-treated Blood.

ABSTRACT

Artificial blood feeding is a common practice for the study and maintenance of blood-feeding arthropod colonies. Commercially purchased blood is often treated to prevent clot-formation using either mechanical or chemical means. For many hematophagous insects, the effect that different anticoagulation methods may have on life history metrics is unclear. In the current study, Culex tarsalis Coquillett and Culicoides sonorensis Wirth & Jones were fed blood treated with either mechanical (defibrination) or chemical (K2 EDTA) anticoagulation methods. Several blood feeding and life history metrics were evaluated between treatment groups including proportion blood feeding, fecundity, fertility, and mortality. No significant differences were found for any of the measured life history metrics for either species. For experiments measuring aspects of these blood feeding and life history traits, blood treated using either defibrination or K2 EDTA anticoagulants should not impact experimental outcomes.