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Vaspin alleviates the lncRNA LEF1-AS1-induced osteogenic differentiation of vascular smooth muscle cells via the Hippo/YAP signaling pathway.
Ma, Xiaoxue; Wang, Yiru; Liu, Qi; Han, Baihe; Wang, Gang; Zhang, Ruoxi; Huang, Xingtao; Wang, Xuedong; Yang, Mengyue; Xing, Chun; Hou, Jingbo; Yu, Bo.
Afiliação
  • Ma X; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Wang Y; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Liu Q; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Han B; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Wang G; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Zhang R; Department of Cardiology, Harbin Yinghua Hospital, Harbin, China.
  • Huang X; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Wang X; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Yang M; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Xing C; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • Hou J; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address: jingbohou@163.com.
  • Yu B; The Key Laboratory of Myocardial Ischemia Organization, Chinese Ministry of Education, Harbin, China; Department of Cardiology Organization, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Exp Cell Res ; 421(2): 113407, 2022 12 15.
Article em En | MEDLINE | ID: mdl-36334793
ABSTRACT
Vascular calcification (VC) is closely related to higher cardiovascular mortality and morbidity, and vascular smooth muscle cell (VSMC) switching to osteogenic-like cells is crucial for VC. LncRNA LEF1-AS1 promotes atherosclerosis and dental pulp stem cells calcification, while its role in VC remains unknown. Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an adipokine regulating bone metabolism. However, the relationship between vaspin and VC is still unclear. We aimed to explore the role of LEF1-AS1 on VSMC osteogenic transition, whether vaspin inhibited LEF1-AS1-mediated osteogenic differentiation of VSMCs, and the responsible mechanism. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis indicated that LEF1-AS1 overexpression significantly upregulated osteogenic marker Runt-related transcription factor-2 (RUNX2) level and downregulated VSMC contractile marker α-smooth muscle actin (α-SMA) level. Alizarin red staining, alkaline phosphatase (ALP) staining, ALP activity assay, and calcium content assay also suggested that LEF1-AS1 overexpression promoted calcium deposition in VSMCs. However, vaspin treatment abolished this phenomenon. Mechanistically, LEF1-AS1 markedly decreased phosphorylated YAP level, while vaspin reversed LEF1-AS1-induced phosphorylated YAP decline. Our results revealed that LEF1-AS1 accelerated the osteogenic differentiation of VSMCs by regulating the Hippo/YAP pathway, while vaspin eliminated the LEF1-AS1-meditated VSMCs osteogenic phenotype switch.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calcificação Vascular / RNA Longo não Codificante Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Calcificação Vascular / RNA Longo não Codificante Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article