Epitope mapping of a blood-brain barrier crossing antibody targeting the cysteine-rich region of IGF1R using hydrogen-exchange mass spectrometry enabled by electrochemical reduction.

Sheff, Joey; Kelly, John; Foss, Mary; Brunette, Eric; Kemmerich, Kristin; van Faassen, Henk; Raphael, Shalini; Hussack, Greg; Comamala, Gerard; Rand, Kasper; Stanimirovic, Danica B

Sheff, Joey; Kelly, John; Foss, Mary; Brunette, Eric; Kemmerich, Kristin; van Faassen, Henk; Raphael, Shalini; Hussack, Greg; Comamala, Gerard; Rand, Kasper; Stanimirovic, Danica B.

Afiliação

Sheff J; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Kelly J; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Foss M; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Brunette E; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Kemmerich K; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
van Faassen H; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Raphael S; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Hussack G; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.
Comamala G; Department of Pharmacy, University of Copenhagen, 2100, Copenhagen, Denmark.2100.
Rand K; Department of Pharmacy, University of Copenhagen, 2100, Copenhagen, Denmark.2100.
Stanimirovic DB; Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, Ontario K1A 0R6, Canada.

J Biochem ; 173(2): 95-105, 2023 Feb 03.

Article em En | MEDLINE | ID: mdl-36346120

Assuntos

Cisteína; Hidrogênio; Mapeamento de Epitopos/métodos; Barreira Hematoencefálica/metabolismo; Anticorpos Monoclonais; Epitopos; Espectrometria de Massas/métodos

Palavras-chave

IGF1R; bloodbrain barrier; epitope mapping; hydrogendeuterium exchange mass spectrometry; single-domain antibody

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cisteína / Hidrogênio Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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