Sometimes faster can be better: Microneedling IPG strips enables higher throughput for integrative top-down proteomics.
Proteomics
; 23(2): e2200307, 2023 01.
Article
em En
| MEDLINE
| ID: mdl-36349823
ABSTRACT
Passive rehydration of immobilized pH gradient (IPG) strips for two-dimensional gel electrophoresis (2DE) has, to our knowledge, never been quantitatively evaluated to determine an ideal rehydration time. Seeking to increase throughput without sacrificing analytical rigor, we report that a substantially shorter rehydration time is accomplished when surface area of IPG strips is increased via microneedling. Rehydration for 4 h, post microneedling, provides comparable results to overnight rehydration in final analyses by 2DE, while also shortening the overall protocol by 1 day.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteômica
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article