A Simplified Approach to Evaluating T Cell Development by Flow Cytometry.
Methods Mol Biol
; 2580: 131-149, 2023.
Article
em En
| MEDLINE
| ID: mdl-36374454
ABSTRACT
T cell development is a complex multistep process that requires the coordinated activation of distinct signaling responses and the regulated progression of developing cells (thymocytes) through key stages of maturation. Although sophisticated techniques such as fetal thymus organ culture, in vitro thymocyte culture, and multiparameter flow cytometry-based cell sorting are now widely employed to evaluate thymocyte maturation by experienced laboratories, defects in T cell development can usually be identified with relatively simple flow cytometry screening methods. Here, we provide a basic protocol for assessment of T cell development that will enable laboratories with access to a multi-laser flow cytometer to screen mouse strains, including those generated from embryonic stem cells with targeted gene mutations, for thymocyte maturation defects.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Timócitos
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article