Studying T Cell Development in Neonatal and Adult Thymic Slices.
Methods Mol Biol
; 2580: 233-247, 2023.
Article
em En
| MEDLINE
| ID: mdl-36374461
ABSTRACT
T cell development occurs in the thymus and is coordinated temporally and spatially within the highly complex thymic microenvironment. Therefore, T cell selection and maturation events cannot be fully recapitulated using traditional two-dimensional tissue culture in vitro. The thymic slice system provides a highly versatile system for studying T cell development ex vivo while preserving three-dimensional thymic architecture. Using the thymic slice system, T cell selection and maturation events can be visualized by live imaging and quantified by flow cytometry. Here we describe the process for generating slices from neonatal and adult mice.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Linfócitos T
Limite:
Animals
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article