An Infectious Virus-like Particle Built on a Programmable Icosahedral DNA Framework.
Angew Chem Int Ed Engl
; 62(4): e202214731, 2023 01 23.
Article
em En
| MEDLINE
| ID: mdl-36377708
ABSTRACT
Viral genomes can be compressed into a near-spherical nanochamber to form infectious particles. In order to mimic the virus morphology and packaging behavior, we invented a programmable icosahedral DNA nanoframe with enhanced rigidity and encapsulated the phiX174 bacteriophage genome. The packaging efficiency could be modulated through specific anchoring strands adjustment, and the trapped phage genome remained accessible for enzymatic operations. Moreover, the packed complex could infect Escherichia coli (E.â
coli) cells through bacterial uptake to produce plaques. This rigid icosahedral DNA architecture demonstrated a versatile platform to develop virus mimetic particles for convenient functional nucleic acid entrapment, manipulation and delivery.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacteriófagos
/
Escherichia coli
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article