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An Infectious Virus-like Particle Built on a Programmable Icosahedral DNA Framework.
Xu, Yunyun; Yang, Yuhe R; Shi, Qian; Ward, Andrew B; Huang, Kui; Chen, Xiao; Wang, Wei; Yang, Yang.
Afiliação
  • Xu Y; School of Medicine, Shanghai Jiao Tong University, Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, 1630 Dongfang Road, B17-1820, Pudong, Shanghai, 200127, P.R. China.
  • Yang YR; National Center for Nanoscience and Technology, Chinese Academy of Sciences, No. 11 ZhongGuanCun BeiYiTiao, Beijing, 100190, P.R. China.
  • Shi Q; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Ward AB; School of Medicine, Shanghai Jiao Tong University, Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, 1630 Dongfang Road, B17-1820, Pudong, Shanghai, 200127, P.R. China.
  • Huang K; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Chen X; School of Medicine, Shanghai Jiao Tong University, Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, 1630 Dongfang Road, B17-1820, Pudong, Shanghai, 200127, P.R. China.
  • Wang W; School of Medicine, Shanghai Jiao Tong University, Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, 1630 Dongfang Road, B17-1820, Pudong, Shanghai, 200127, P.R. China.
  • Yang Y; School of Medicine, Shanghai Jiao Tong University, Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, 1630 Dongfang Road, B17-1820, Pudong, Shanghai, 200127, P.R. China.
Angew Chem Int Ed Engl ; 62(4): e202214731, 2023 01 23.
Article em En | MEDLINE | ID: mdl-36377708
ABSTRACT
Viral genomes can be compressed into a near-spherical nanochamber to form infectious particles. In order to mimic the virus morphology and packaging behavior, we invented a programmable icosahedral DNA nanoframe with enhanced rigidity and encapsulated the phiX174 bacteriophage genome. The packaging efficiency could be modulated through specific anchoring strands adjustment, and the trapped phage genome remained accessible for enzymatic operations. Moreover, the packed complex could infect Escherichia coli (E. coli) cells through bacterial uptake to produce plaques. This rigid icosahedral DNA architecture demonstrated a versatile platform to develop virus mimetic particles for convenient functional nucleic acid entrapment, manipulation and delivery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacteriófagos / Escherichia coli Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacteriófagos / Escherichia coli Idioma: En Ano de publicação: 2023 Tipo de documento: Article