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Site-directed mutagenesis of HLA molecules reveals the functional epitope of a human HLA-A1/A36-specific monoclonal antibody.
Meng, Tina; Bezstarosti, Suzanne; Singh, Ujjwala; Yap, Michelle; Scott, Laura; Petrosyan, Naiiry; Quiroz, Fred; Eps, Ned Van; Hui, Eric Ka-Wai; Suh, David; Zhu, Quansheng; Pei, Rui; Kramer, Cynthia S M; Claas, Frans H J; Lowe, David; Heidt, Sebastiaan.
Afiliação
  • Meng T; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Bezstarosti S; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
  • Singh U; Department of Internal Medicine (Nephrology), Leiden University Medical Center, Leiden, The Netherlands.
  • Yap M; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Scott L; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Petrosyan N; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Quiroz F; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Eps NV; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Hui EK; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Suh D; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Zhu Q; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Pei R; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Kramer CSM; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
  • Claas FHJ; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
  • Lowe D; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands.
  • Heidt S; Department of Research and Development, One Lambda, Inc. (A Part of Thermo Fisher Scientific Inc.), West Hills, California, USA.
HLA ; 101(2): 138-142, 2023 02.
Article em En | MEDLINE | ID: mdl-36401817
ABSTRACT
Eplet 44KM is currently listed in the HLA Epitope Registry but does not adhere to the eplet definition of an amino acid configuration within a 3.5 Å radius. Eplet 44KM has been previously redefined to the antibody-verified reactivity pattern 44K/150V/158V, based on reactivity analysis of monoclonal antibody VDK1D12. Since the three residues are always simultaneously present on common HLA alleles, methods to define which residue is crucial for antibody-induction and binding are limited. In this proof-of-concept study, we performed site-directed mutagenesis to narrow down the antibody-verified reactivity pattern 44K/150V/158V to a single amino acid and defined 44K as the eplet or functional epitope of mAb VDK1D12.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígeno HLA-A1 / Anticorpos Monoclonais Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígeno HLA-A1 / Anticorpos Monoclonais Limite: Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article