Persistent ATP-Concentration Gradients in a Hydrogel Sustained by Chemical Fuel Consumption.
Angew Chem Int Ed Engl
; 62(4): e202215421, 2023 01 23.
Article
em En
| MEDLINE
| ID: mdl-36420591
ABSTRACT
We show the formation of macroscopic ATP-concentrations in an agarose gel and demonstrate that these gradients can be sustained in time at the expense of the consumption of a chemical fuel. The approach relies on the spatially controlled activation of ATP-producing and ATP-consuming reactions through the local injection of enzymes in the matrix. The reaction-diffusion system is maintained in a stationary non-equilibrium state as long as chemical fuel, phosphocreatine, is present. The reaction-diffusion system is coupled to a supramolecular system composed of monolayer protected gold nanoparticles and a fluorescent probe. As a result of this coupling, fluorescence signals emerge spontaneously in response to the ATP-concentration gradients. We show that the approach permits the rational formation of complex fluorescence patterns that change over time as a function of the evolution of the ATP-concentrations present in the system.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrogéis
/
Nanopartículas Metálicas
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article