Detergent-Assisted Protein Digestion-On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics.

Danko, Katerina; Lukasheva, Elena; Zhukov, Vladimir A; Zgoda, Viktor; Frolov, Andrej

Danko, Katerina; Lukasheva, Elena; Zhukov, Vladimir A; Zgoda, Viktor; Frolov, Andrej.

Afiliação

Danko K; Department of Biochemistry, St. Petersburg State University, 199034 St. Petersburg, Russia.
Lukasheva E; Department of Biochemistry, St. Petersburg State University, 199034 St. Petersburg, Russia.
Zhukov VA; All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chaussee 3, Pushkin, 196608 St. Petersburg, Russia.
Zgoda V; Institute of Biomedical Chemistry, 119121 Moscow, Russia.
Frolov A; K.A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia.

Int J Mol Sci ; 23(22)2022 Nov 11.

Article em En | MEDLINE | ID: mdl-36430380

Assuntos

Detergentes; Proteômica; Proteômica/métodos; Proteólise; Espectrometria de Massas em Tandem; Proteoma; Peptídeos/química

Palavras-chave

bottom-up proteomics; detergent-assisted proteolysis; detergents; filter-assisted sample preparation (FASP); gel-free proteomics; shotgun proteomics; sodium dodecyl sulfate (SDS); surfactants

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteômica / Detergentes Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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