Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.
Methods Mol Biol
; 2595: 171-183, 2023.
Article
em En
| MEDLINE
| ID: mdl-36441462
ABSTRACT
Single microRNA (miRNA) can be inhibited using antagomiR which efficiently knocks down a specific miRNA. However, the effect is transient and often results in subtle phenotype. Here we report a guideline on designing miRNA sponges inhibiting a miRNA family. As a model system, we targeted miR-30 family, known as tumor suppressor miRNAs in multiple tumors. To achieve an efficient knockdown, we generated perfect and bulged-matched miRNA binding sites (MBS) and introduced multiple copies of MBS. The protocol here demonstrates the miRNA sponge as a useful tool to examine the functional impact of inhibition miRNAs.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article