Cytochromeâ
P450 Catalyzes Benzene Ring Formation in the Biosynthesis of Trialkyl-Substituted Aromatic Polyketides.
Angew Chem Int Ed Engl
; 62(5): e202214026, 2023 01 26.
Article
em En
| MEDLINE
| ID: mdl-36458944
ABSTRACT
Lorneic acid and related natural products are characterized by a trialkyl-substituted benzene ring. The formation of the aromatic core in the middle of the polyketide chain is unusual. We characterized a cytochromeâ
P450 enzyme that can catalyze the hallmark benzene ring formation from an acyclic polyene substrate through genetic and biochemical analysis. Using this P450 as a beacon for genome mining, we obtained 12 homologous type I polyketide synthase (PKS) gene clusters, among which two gene clusters are activated and able to produce trialkyl-substituted aromatic polyketides. Quantum chemical calculations were performed to elucidate the plausible mechanism for P450-catalyzed benzene ring formation. Our work expands our knowledge of the catalytic diversity of cytochromeâ
P450.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Policetídeos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article