Purification of individual tRNAs using a monoclonal anti-AMP antibody affinity column.
Anal Biochem
; 161(2): 460-6, 1987 Mar.
Article
em En
| MEDLINE
| ID: mdl-3646854
ABSTRACT
A murine monoclonal anti-AMP antibody affinity matrix was used for isolation of individual species of amino acid transfer nucleic acids (tRNAs). The antibodies had been prepared using 5'-AMP covalently attached to bovine serum albumin as antigen and exhibited high affinity for 5'-AMP but greatly reduced affinity for 3'-AMP. Native uncharged tRNAs that terminate in a 5'-AMP group on the amino acid acceptor arm of the molecule bind tightly to the anti-AMP affinity matrix, whereas aminoacylated tRNAs are not retained. This allows separation of a particular tRNA species as its aminoacyl derivative from a complex mixture of uncharged tRNAs under very mild conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência
/
Monofosfato de Adenosina
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article