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Assessment of diversity of archaeal communities in Algerian chott.
Hassani, Imene Ikram; Quadri, Inès; Yadav, Archana; Bouchard, Sonia; Raoult, Didier; Hacène, Hocine; Desnues, Christelle.
Afiliação
  • Hassani II; Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Biologie, USTHB Université, Bab Ezzouar, Algeria. hassani_ikram@yahoo.fr.
  • Quadri I; Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Biologie, USTHB Université, Bab Ezzouar, Algeria.
  • Yadav A; Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA.
  • Bouchard S; Faculté de Médecine, Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD 198, Inserm U1095, 27 Boulevard Jean Moulin, 13385, Marseille, France.
  • Raoult D; Faculté de Médecine, Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD 198, Inserm U1095, 27 Boulevard Jean Moulin, 13385, Marseille, France.
  • Hacène H; Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Biologie, USTHB Université, Bab Ezzouar, Algeria.
  • Desnues C; Faculté de Médecine, Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD 198, Inserm U1095, 27 Boulevard Jean Moulin, 13385, Marseille, France.
Extremophiles ; 27(1): 2, 2022 Dec 05.
Article em En | MEDLINE | ID: mdl-36469177
Halophilic archaea are the dominant type of microorganisms in hypersaline environments. The diversity of halophilic archaea in Zehrez-Chergui (Saharian chott) was analyzed and compared by both analysis of a library of PCR amplified 16S rRNA genes and by cultivation approach. This work, represents the first of its type in Algeria. A total cell count was estimated at 3.8 × 103 CFU/g. The morphological, biochemical, and physiological characterizations of 45 distinct strains, suggests that all of them might be members of the class Halobacteria. Among stains, 23 were characterized phylogenetically and are related to 6 genera of halophilic archaea.The dominance of the genus Halopiger, has not been reported yet in other hypersaline environments. The 100 clones obtained by the molecular approach, were sequenced, and analyzed. The ribosomal library of 61 OTUs showed that the archaeal diversity included uncultured haloarcheon, Halomicrobium, Natronomonas, Halomicroarcula, Halapricum, Haloarcula, Halosimplex, Haloterrigena, Halolamina, Halorubellus, Halorussus and Halonotius. The results of rarefaction analysis indicated that the analysis of an increasing number of clones would have revealed additional diversity. Surprisingly, no halophilic archaea were not shared between the two approaches. Combining both types of methods was considered the best approach to acquire better information on the characteristics of soil halophilic archaea.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Halobacteriales / Euryarchaeota País/Região como assunto: Africa Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Halobacteriales / Euryarchaeota País/Região como assunto: Africa Idioma: En Ano de publicação: 2022 Tipo de documento: Article