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Quantitative imaging of single-cell phenotypes in cancer cells cultured on hydrogel surfaces.
Bousgouni, Vicky; Bakal, Chris.
Afiliação
  • Bousgouni V; Dynamical Cell Systems Laboratory, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. Electronic address: vicky.bousgouni@icr.ac.uk.
  • Bakal C; Dynamical Cell Systems Laboratory, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. Electronic address: chris.bakal@icr.ac.uk.
STAR Protoc ; 4(1): 101942, 2023 03 17.
Article em En | MEDLINE | ID: mdl-36525347
Small interfering RNA (siRNA) screening approaches used with quantitative single-cell analysis can uncover the roles of genes in cell morphogenesis. Here, we present a high-throughput automated phenotypic screening technique to quantify a single cell shape in cancer cells cultured on top of soft 3D hydrogels. We describe reverse transfection of cells with siRNAs and seeding of these cells on top of collagen, followed by image analysis to quantify morphology of a single cell and population levels in low-elasticity matrices. For complete details on the use and execution of this protocol, please refer to Bousgouni et al. (2022).1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrogéis / Neoplasias Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrogéis / Neoplasias Idioma: En Ano de publicação: 2023 Tipo de documento: Article