A novel strand-specific RNA-sequencing protocol using dU-adaptor-assembled Tn5.

Tao, Xiaoyuan; Feng, Shouli; Li, Sujuan; Chen, Guang; Wang, Jian; Xu, Lizhi; Fu, Xujun; Yu, Jing; Xu, Shengchun

Tao, Xiaoyuan; Feng, Shouli; Li, Sujuan; Chen, Guang; Wang, Jian; Xu, Lizhi; Fu, Xujun; Yu, Jing; Xu, Shengchun.

Afiliação

Tao X; Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.
Feng S; College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, P. R. China.
Li S; Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.
Chen G; Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.
Wang J; Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.
Xu L; Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.
Fu X; Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.
Yu J; Zhejiang Future Gene Bio-technology Company, Jingning, 323500, P. R. China.
Xu S; Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P. R. China.

J Exp Bot ; 74(6): 1806-1820, 2023 03 28.

Article em En | MEDLINE | ID: mdl-36585802

Assuntos

Cromatina; Perfilação da Expressão Gênica; Biblioteca Gênica; DNA Complementar/genética; Perfilação da Expressão Gênica/métodos; Análise de Sequência de RNA/métodos; Sequenciamento de Nucleotídeos em Larga Escala/métodos; RNA

Palavras-chave

Tn5; dU-Tn5; dUTP; lncRNA; soybean; stranded RNA-seq

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatina / Perfilação da Expressão Gênica Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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