Integrated slip valve-assisted fluidic chip coupling with CRISPR/Cas12a system for nucleic acid analysis.

Qian, Siwenjie; Chen, Yanju; Peng, Cheng; Wang, Xiaofu; Che, Yang; Wang, Tingzhang; Wu, Jian; Xu, Junfeng

Qian, Siwenjie; Chen, Yanju; Peng, Cheng; Wang, Xiaofu; Che, Yang; Wang, Tingzhang; Wu, Jian; Xu, Junfeng.

Afiliação

Qian S; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
Chen Y; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
Peng C; Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Wang X; Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Che Y; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, 310012, China.
Wang T; Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, 310012, China. Electronic address: wtz8615@tkgeneclub.com.
Wu J; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of on Site Processing Equipment for Agricultural Products, Ministry of Agriculture and Rural Affairs, Hangzhou, 310058, China. Electronic address: wujian69@zju.edu.cn.
Xu J; Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. Electronic address: xujunfeng@zaas.ac.cn.

Anal Chim Acta ; 1239: 340670, 2023 Jan 25.

Article em En | MEDLINE | ID: mdl-36628703

Assuntos

Sistemas CRISPR-Cas; Ácidos Nucleicos; Técnicas de Amplificação de Ácido Nucleico/métodos; Salmonella typhimurium

Palavras-chave

CRISPR/Cas12a; Dual detection; Integrated chip; Loop-mediated isothermal amplification; Nucleic acid detection

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Sistemas CRISPR-Cas Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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