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Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry.
Quigley, Luke T; Pang, Lokman; Tavancheh, Elnaz; Ernst, Matthias; Behren, Andreas; Huynh, Jennifer; Da Gama Duarte, Jessica.
Afiliação
  • Quigley LT; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
  • Pang L; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
  • Tavancheh E; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
  • Ernst M; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
  • Behren A; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia; Department of Medicine, University of Melbourne, Parkville, VIC 3010, Australia.
  • Huynh J; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
  • Da Gama Duarte J; Olivia Newton-John Cancer Research Institute, and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia. Electronic address: jessica.duarte@onjcri.org.au.
STAR Protoc ; 4(1): 101961, 2023 03 17.
Article em En | MEDLINE | ID: mdl-36633948
Tertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates that can develop within or adjacent to tumors, but protocols that can accurately identify and characterize TLSs are lacking. Here, we present a protocol for the in situ interrogation and characterization of TLSs in human and murine tissue sections using Opal™-tyramide signal amplification multiplex immunohistochemistry. This protocol enables simultaneous detection of up to 7 markers (6 antigens and a DAPI counterstain). We also describe a grading system to identify immature and mature TLSs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estruturas Linfoides Terciárias / Neoplasias Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estruturas Linfoides Terciárias / Neoplasias Limite: Animals / Humans Idioma: En Ano de publicação: 2023 Tipo de documento: Article