Fish Oil - Omega-3 Exerts Protective Effect in Oxidative Stress and Liver Dysfunctions Resulting from Experimental Sepsis.
J Clin Exp Hepatol
; 13(1): 64-74, 2023.
Article
em En
| MEDLINE
| ID: mdl-36647406
ABSTRACT
Background:
Sepsis is a severe global health problem, with high morbidity and mortality. In sepsis, one of the main affected organs is the liver. Hepatic alterations characterize a negative prognostic. Omega-3 fatty acids (ω3), eicosapentaenoic acid, and docosahexaenoic acid, are part of the main families of polyunsaturated fatty acids. ω3 has been used in studies as sepsis treatment and as a treatment for non-alcoholic liver disease.Aim:
We aimed to evaluate the effects of treatment with fish oil (FO) rich in ω3 on liver changes and damage resulting from experimental sepsis.Methodology:
A model of severe sepsis in Wistar rats was used. Oxidative stress in the liver tissue was evaluated by means of tests of thiobarbituric acid reactive substances, 2,7-dihydrodichlorofluorescein diacetate , catalase, and glutathione peroxidase, in the serum TBARS, DCF, thiols and, to assess liver dysfunction, alanine aminotransferase and aspartate aminotransferase. Hepatic tissue damage was evaluated using H&E histology.Results:
In assessments of oxidative stress in liver tissue, a protective effect was observed in the tests of TBARS, DCF, CAT, and GPx, when compared the sepsis versus sepsis+ω3 groups. Regarding the oxidative stress in serum, a protective effect of treatment with ω3 was observed in the TBARS, DCF, and thiols assays, in the comparison between the sepsis and sepsis+ω3 groups. ω3 had also a beneficial effect on biochemical parameters in serum in the analysis of ALT, creatinine, urea, and lactate, observed in the comparison between the sepsis and sepsis+ω3 groups.Conclusion:
The results suggest ω3 as a liver protector during sepsis with an antioxidant effect, alleviating injuries and dysfunctions.
ALT, alanine aminotransferase; AST, aspartate aminotransferase; CAT, catalase; DCF, 2,7-dihydrodichlorofluorescein diacetate; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; FO, fish oil; GPx, glutathione peroxidase; GTO, oxaloacetic transaminase; GTP, pyruvic transaminase; HE, Hematoxylin and Eosin; ICON, Intensive Care Over Nations; ICU, intensive care unit; IFN- γ, interferon gamma; Liver injury; RNS, reactive nitrogen species; ROS, reactive oxygen species; TBARS, Thiobarbituric Acid Reactive Substances; TGF-ß, transforming growth factor beta; TNF-α, tumor necrosis factor alpha; antioxidant; inflammation; omega-3; oxidative stress; sepsis; ω3, omega-3
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article