Identification of two variants of troponin T in the developing chicken heart using a monoclonal antibody.
J Biochem
; 102(1): 25-30, 1987 Jul.
Article
em En
| MEDLINE
| ID: mdl-3667565
ABSTRACT
Troponin T (TNT) expressed in the developing chicken cardiac muscle was examined by immunoblotting combined with two-dimensional electrophoresis (2-D PAGE) and peptide mapping. When the whole lysate of the neonatal heart was examined by 2-D PAGE, two TNT variants were detected on the gel by monoclonal antibody to TNT. Expression of the two variants was developmentally regulated one isoform (type I) was expressed from embryonic through neonatal stages, and the other (type II) from the late embryonic stage through adulthood during cardiac muscle development. The type-I isoform, but not type-II isoform, was also expressed transiently in chicken skeletal muscle at embryonic stages. As judged from the peptide maps, the two isoforms differed in the N-terminal region but not in the C-terminal region.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Troponina
/
Variação Genética
/
Coração
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article