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Eukaryotic translation initiation factor eIF4E-5 is required for spermiogenesis in Drosophila melanogaster.
Shao, Lisa; Fingerhut, Jaclyn M; Falk, Brook L; Han, Hong; Maldonado, Giovanna; Qiao, Yuemeng; Lee, Vincent; Hall, Elizabeth; Chen, Liang; Polevoy, Gordon; Hernández, Greco; Lasko, Paul; Brill, Julie A.
Afiliação
  • Shao L; Cell Biology Program, The Hospital for Sick Children, PGCRL Building, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada.
  • Fingerhut JM; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, M5S 1A8, Canada.
  • Falk BL; Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, 455 Main Street, Cambridge, MA 02142, USA.
  • Han H; Howard Hughes Medical Institute, 455 Main Street, Cambridge, MA 02142, USA.
  • Maldonado G; Cell Biology Program, The Hospital for Sick Children, PGCRL Building, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada.
  • Qiao Y; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, M5S 1A8, Canada.
  • Lee V; Department of Biology, McGill University, 3649 Promenade Sir William Osler, Montréal, Quebec, H3G 0B1, Canada.
  • Hall E; Laboratory of Translation and Cancer, Unit of Biomedical Research on Cancer, Instituto Nacional de Cancerología (INCan), Av San Fernando 22, Mexico City 14080, Mexico.
  • Chen L; Cell Biology Program, The Hospital for Sick Children, PGCRL Building, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada.
  • Polevoy G; Human Biology Program, University of Toronto, 300 Huron Street, Toronto, Ontario, M5S 3J6, Canada.
  • Hernández G; Cell Biology Program, The Hospital for Sick Children, PGCRL Building, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada.
  • Lasko P; Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, M5S 1A8, Canada.
  • Brill JA; Cell Biology Program, The Hospital for Sick Children, PGCRL Building, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada.
Development ; 150(4)2023 02 15.
Article em En | MEDLINE | ID: mdl-36695474
Drosophila sperm development is characterized by extensive post-transcriptional regulation whereby thousands of transcripts are preserved for translation during later stages. A key step in translation initiation is the binding of eukaryotic initiation factor 4E (eIF4E) to the 5' mRNA cap. In addition to canonical eIF4E-1, Drosophila has multiple eIF4E paralogs, including four (eIF4E-3, -4, -5, and -7) that are highly expressed in the testis. Among these, only eIF4E-3 has been characterized genetically. Here, using CRISPR/Cas9 mutagenesis, we determined that eIF4E-5 is essential for male fertility. eIF4E-5 protein localizes to the distal ends of elongated spermatid cysts, and eIF4E-5 mutants exhibit defects during post-meiotic stages, including a mild defect in spermatid cyst polarization. eIF4E-5 mutants also have a fully penetrant defect in individualization, resulting in failure to produce mature sperm. Indeed, our data indicate that eIF4E-5 regulates non-apoptotic caspase activity during individualization by promoting local accumulation of the E3 ubiquitin ligase inhibitor Soti. Our results further extend the diversity of non-canonical eIF4Es that carry out distinct spatiotemporal roles during spermatogenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sêmen / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sêmen / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2023 Tipo de documento: Article